Polarization-dependent fluorescence correlation spectroscopy for studying structural properties of proteins in living cell.

ABSTRACT: Rotational diffusion measurement is predicted as an important method in cell biology because the rotational properties directly reflect molecular interactions and environment in the cell. To prove this concept, polarization-dependent fluorescence correlation spectroscopy (pol-FCS) measurements of purified fluorescent proteins were conducted in viscous solution. With the comparison between the translational and rotational diffusion coefficients obtained from pol-FCS measurements, the hydrodynamic radius of an enhanced green fluorescent protein (EGFP) was estimated as a control measurement. The orientation of oligomer EGFP in living cells was also estimated by pol-FCS and compared with Monte Carlo simulations. The results of this pol-FCS experiment indicate that this method allows an estimat

SUBMITTER: Oura M 

PROVIDER: S-EPMC4973283 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature