A high-resolution transcriptome map of cell cycle reveals novel connections between periodic genes and cancer.

ABSTRACT: Progression through the cell cycle is largely dependent on waves of periodic gene expression, and the regulatory networks for these transcriptome dynamics have emerged as critical points of vulnerability in various aspects of tumor biology. Through RNA-sequencing of human cells during two continuous cell cycles (>2.3 billion paired reads), we identified over 1 000 mRNAs, non-coding RNAs and pseudogenes with periodic expression. Periodic transcripts are enriched in functions related to DNA metabolism, mitosis, and DNA damage response, indicating these genes likely represent putative cell cycle regulators. Using our set of periodic genes, we developed a new approach termed "mitotic trait" that can classify primary tumors and normal tissues by their transcriptome similarity to different cell

SUBMITTER: Dominguez D 

PROVIDER: S-EPMC4973334 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature