Forward genetic screen of human transposase genomic rearrangements.

ABSTRACT:

Background

Numerous human genes encode potentially active DNA transposases or recombinases, but our understanding of their functions remains limited due to shortage of methods to profile their activities on endogenous genomic substrates.

Results

To enable functional analysis of human transposase-derived genes, we combined forward chemical genetic hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) screening with massively parallel paired-end DNA sequencing and structural variant genome assembly and analysis. Here, we report the HPRT1 mutational spectrum induced by the human transposase PGBD5, including PGBD5-specific signal sequences (PSS) that serve as potential genomic rearrangement substrates.

Conclusions

The discovered PSS motifs and high-throughput forward c

SUBMITTER: Henssen AG 

PROVIDER: S-EPMC4973553 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature