Cell Surface Proteome of Dental Pulp Stem Cells Identified by Label-Free Mass Spectrometry.

ABSTRACT: Multipotent mesenchymal stromal cells (MSCs) are promising tools for regenerative medicine. They can be isolated from different sources based on their plastic-adherence property. The identification of reliable cell surface markers thus becomes the Holy Grail for their prospective isolation. Here, we determine the cell surface proteomes of human dental pulp-derived MSCs isolated from single donors after culture expansion in low (2%) or high (10%) serum-containing media. Cell surface proteins were tagged on intact cells using cell impermeable, cleavable sulfo-NHS-SS-biotin, which allows their enrichment by streptavidin pull-down. For the proteomic analyses, we first compared label-free methods to analyze cell surface proteomes i.e. composition, enrichment and proteomic differences, and we de

SUBMITTER: Niehage C 

PROVIDER: S-EPMC4973913 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature