Genetically encoded protein photocrosslinker with a transferable mass spectrometry-identifiable label.

ABSTRACT: Coupling photocrosslinking reagents with mass spectrometry has become a powerful tool for studying protein-protein interactions in living systems, but it still suffers from high rates of false-positive identifications as well as the lack of information on interaction interface due to the challenges in deciphering crosslinking peptides. Here we develop a genetically encoded photo-affinity unnatural amino acid that introduces a mass spectrometry-identifiable label (MS-label) to the captured prey proteins after photocrosslinking and prey-bait separation. This strategy, termed IMAPP (In-situ cleavage and MS-label transfer After Protein Photocrosslinking), enables direct identification of photo-captured substrate peptides that are difficult to uncover by conventional genetically encoded photocr

SUBMITTER: Yang Y 

PROVIDER: S-EPMC4974458 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature