Antimicrobial peptides trigger a division block in Escherichia coli through stimulation of a signalling system.

ABSTRACT: Antimicrobial peptides are an important component of the molecular arsenal employed by hosts against bacteria. Many bacteria in turn possess pathways that provide protection against these compounds. In Escherichia coli and related bacteria, the PhoQ/PhoP signalling system is a key regulator of this antimicrobial peptide defence. Here we show that treating E. coli with sublethal concentrations of antimicrobial peptides causes cells to filament, and that this division block is controlled by the PhoQ/PhoP system. The filamentation results from increased expression of QueE, an enzyme that is part of a tRNA modification pathway but that, as we show here, also affects cell division. We also find that a functional YFP-QueE fusion localizes to the division septum in filamentous cells, suggesting Q

SUBMITTER: Yadavalli SS 

PROVIDER: S-EPMC4974570 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature