Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.

ABSTRACT: Current biopharmaceutical manufacturing systems are not compatible with portable or distributed production of biologics, as they typically require the development of single biologic-producing cell lines followed by their cultivation at very large scales. Therefore, it remains challenging to treat patients in short time frames, especially in remote locations with limited infrastructure. To overcome these barriers, we developed a platform using genetically engineered Pichia pastoris strains designed to secrete multiple proteins on programmable cues in an integrated, benchtop, millilitre-scale microfluidic device. We use this platform for rapid and switchable production of two biologics from a single yeast strain as specified by the operator. Our results demonstrate selectable and near-single

SUBMITTER: Perez-Pinera P 

PROVIDER: S-EPMC4974573 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature