Quantification of Hepatic Vascular and Parenchymal Regeneration in Mice.

ABSTRACT:

Background

Liver regeneration consists of cellular proliferation leading to parenchymal and vascular growth. This study complements previous studies on cellular proliferation and weight recovery by (1) quantitatively describing parenchymal and vascular regeneration, and (2) determining their relationship. Both together are needed to (3) characterize the underlying growth pattern.

Methods

Specimens were created by injecting a polymerizing contrast agent in either portal or hepatic vein in normal or regenerating livers after 70% partial hepatectomy. 3D image data were obtained through micro-CT scanning. Parenchymal growth was assessed by determining weight and volume of the regenerating liver. Vascular growth was described by manually determined circumscribed parameters (maxim

SUBMITTER: Xie C 

PROVIDER: S-EPMC4975469 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature