Development of two PCR-based techniques for detecting helical and coccoid forms of Helicobacter pylori.

ABSTRACT: The primary mode of transmission of Helicobacter pylori, a human pathogen carried by more than half the population worldwide, is still unresolved. Some epidemiological data suggest water as a possible transmission route. H. pylori in the environment transforms into a nonculturable, coccoid form, which frequently results in the failure to detect this bacterium in environmental samples by conventional culture techniques. To overcome limitations associated with culturing, molecular approaches based on DNA amplification by PCR have been developed and used for the detection of H. pylori in clinical and environmental samples. Our results showed the glmM gene as the most promising target for detection of H. pylori by PCR amplification. Under optimal amplification conditions, glmM-specific primers

SUBMITTER: Shahamat M 

PROVIDER: S-EPMC497564 | biostudies-literature | 2004 Aug

REPOSITORIES: biostudies-literature