MiR-27b inhibits LDLR and ABCA1 expression but does not influence plasma and hepatic lipid levels in mice.

ABSTRACT:

Rationale

Recently, there has been significant interest in the therapeutic administration of miRNA mimics and inhibitors to treat cardiovascular disease. In particular, miR-27b has emerged as a regulatory hub in cholesterol and lipid metabolism and potential therapeutic target for treating atherosclerosis. Despite this, the impact of miR-27b on lipid levels in vivo remains to be determined. As such, here we set out to further characterize the role of miR-27b in regulating cholesterol metabolism in vitro and to determine the effect of miR-27b overexpression and inhibition on circulating and hepatic lipids in mice.

Methods and results

Our results identify miR-27b as an important regulator of LDLR activity in human and mouse hepatic cells through direct targeting of LDLR and LD

SUBMITTER: Goedeke L 

PROVIDER: S-EPMC4975922 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature