Primary Cell Culture Systems for Human Thyroid Studies.

ABSTRACT:

Background

Cell models are key instruments for in vitro studies of the thyroid. Permanent thyroid cell lines that are widely used in laboratory research typically originate from tumors. For many purposes, it is desirable to compare tumor cells with cells originating from normal tissue. However, such cultures grow slowly, have a highly limited life-span, and are known to lose their thyroid characteristics. The aim of the present study was to type coding and noncoding thyroid markers in different culture systems in an attempt to determine the optimal conditions for in vitro experimentation.

Methods

Human primary thyroid cells were isolated from histologically non-tumorous tissues. Two alternative media (6H and h7H) were used. The morphology and behavior of the ensuing monolaye

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4976228 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature