An assay for 26S proteasome activity based on fluorescence anisotropy measurements of dye-labeled protein substrates.

ABSTRACT: The 26S proteasome is the molecular machine at the center of the ubiquitin proteasome system and is responsible for adjusting the concentrations of many cellular proteins. It is a drug target in several human diseases, and assays for the characterization of modulators of its activity are valuable. The 26S proteasome consists of two components: a core particle, which contains the proteolytic sites, and regulatory caps, which contain substrate receptors and substrate processing enzymes, including six ATPases. Current high-throughput assays of proteasome activity use synthetic fluorogenic peptide substrates that report directly on the proteolytic activity of the proteasome, but not on the activities of the proteasome caps that are responsible for protein recognition and unfolding. Here, we de

SUBMITTER: Bhattacharyya S 

PROVIDER: S-EPMC4976823 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature