Human Organotypic Lung Tumor Models: Suitable For Preclinical 18F-FDG PET-Imaging.

ABSTRACT: Development of predictable in vitro tumor models is a challenging task due to the enormous complexity of tumors in vivo. The closer the resemblance of these models to human tumor characteristics, the more suitable they are for drug-development and -testing. In the present study, we generated a complex 3D lung tumor test system based on acellular rat lungs. A decellularization protocol was established preserving the architecture, important ECM components and the basement membrane of the lung. Human lung tumor cells cultured on the scaffold formed cluster and exhibited an up-regulation of the carcinoma-associated marker mucin1 as well as a reduced proliferation rate compared to respective 2D culture. Additionally, employing functional imaging with 2-deoxy-2-[18F]fluoro-D-glucose positron emi

SUBMITTER: Fecher D 

PROVIDER: S-EPMC4976941 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature