A Flow Cytometric and Computational Approaches to Carbapenems Affinity to the Different Types of Carbapenemases.

ABSTRACT: The synergy of carbapenem combinations regarding Enterobacteriaceae producing different types of carbapenemases was study through different approaches: flow cytometry and computational analysis. Ten well characterized Enterobacteriaceae (KPC, verona integron-encoded metallo-β-lactamases -VIM and OXA-48-like enzymes) were selected for the study. The cells were incubated with a combination of ertapenem with imipenem, meropenem, or doripenem and killing kinetic curves performed with and without reinforcements of the drugs. A cephalosporin was also used in combination with ertapenem. A flow cytometric assay with DiBAC4-(3), a membrane potential dye, was developed in order to evaluate the cellular lesion after 2 h incubation. A chemical computational study was performed to understand the affini

SUBMITTER: Pina-Vaz C 

PROVIDER: S-EPMC4977277 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature