18O proteomics reveal increased human apolipoprotein CIII in Hispanic HIV-1+ women with HAART that use cocaine.

ABSTRACT:

Purpose

Drug abuse is a major risk factor in the development and progression of HIV-1. This study defines the alterations in the plasma proteome of HIV-1-infected women that use cocaine.

Experimental design

Plasma samples from 12 HIV-seropositive Hispanic women under antiretroviral therapy were selected for this study. Six sample pairs were matched between nondrug users and cocaine users. After IgG and albumin depletion, SDS-PAGE, and in-gel digestion, peptides from nondrug users and cocaine users were labeled with (16) O and (18) O, respectively, and subjected to LC-MS/MS and quantitation using Proteome Discover and QuiXoT softwares and validated by ELISA.

Results

A total of 1015 proteins were identified at 1% false discovery rates (FDR). Statistical analyses reveale

SUBMITTER: Zenon F 

PROVIDER: S-EPMC4977997 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature