Assessment of the Human Cytomegalovirus UL97 Gene for Identification of Resistance to Ganciclovir in Iranian Immunosuppressed Patients.

ABSTRACT:

Background

Human cytomegalovirus (HCMV) infections are a major cause of morbidity and mortality among immunocompromised patients. Prolonged antiviral therapy is a cause of mutation and drug resistance in the HCMV genome.

Objectives

The aim of this study was to identify resistance to ganciclovir (GCV) in Iranian immunosuppressed patients at two different stages of the disease: early (before GCV is initiated) and late (after six months of GCV therapy).

Patients and methods

In this study, 87 specimens from Iranian patients were amplified using nested PCR amplification of the UL97 gene. Sequence analyses of products were performed for identifying the mutated codons.

Results

The present study show that the most frequent GCV-resistant mutations occurred in codons A59

SUBMITTER: Keyvani H 

PROVIDER: S-EPMC4978088 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature