Live Attenuated Influenza Vaccine in Children Induces B-Cell Responses in Tonsils.

ABSTRACT:

Background

Tonsils play a key role in eliciting immune responses against respiratory pathogens. Little is known about how tonsils contribute to the local immune response after intranasal vaccination. Here, we uniquely report the mucosal humoral responses in tonsils and saliva after intranasal live attenuated influenza vaccine (LAIV) vaccination in children.

Methods

Blood, saliva, and tonsils samples were collected from 39 children before and after LAIV vaccination and from 16 age-matched, nonvaccinated controls. Serum antibody responses were determined by a hemagglutination inhibition (HI) assay. The salivary immunoglobulin A (IgA) level was measured by an enzyme-linked immunosorbent assay. Antibody-secreting cell (ASC) and memory B-cell (MBC) responses were enumerated in to

SUBMITTER: Mohn KG 

PROVIDER: S-EPMC4978372 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature