A Novel Approach to Helicobacter pylori Pan-Genome Analysis for Identification of Genomic Islands.

ABSTRACT: Genomes of a given bacterial species can show great variation in gene content and thus systematic analysis of the entire gene repertoire, termed the pan-genome, is important for understanding bacterial intra-species diversity, population genetics, and evolution. Here, we analyzed the pan-genome from 30 completely sequenced strains of the human gastric pathogen Helicobacter pylori belonging to various phylogeographic groups, focusing on 991 accessory (not fully conserved) orthologous groups (OGs). We developed a method to evaluate the mobility of genes within a genome, using the gene order in the syntenically conserved regions as a reference, and classified the 991 accessory OGs into five classes: Core, Stable, Intermediate, Mobile, and Unique. Phylogenetic networks based on the gene conten

SUBMITTER: Uchiyama I 

PROVIDER: S-EPMC4978471 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature