CpG site methylation in CRYAA promoter affect transcription factor Sp1 binding in human lens epithelial cells.

ABSTRACT:

Background

Age-related cataract (ARC) is the leading cause of visual impairment worldwide, and α-crystallin (CRYAA) is the predominant structural protein involved in the maintenance of lens clarity and refractive properties. We previously demonstrated that CRYAA genes undergo epigenetic repression in the lens epithelia in ARC. We further analyze the underlying mechanism in the current study.

Methods

The transcription factor binding sites of the CpG island of CRYAA promoter were predicted by TESS website. An electrophoretic mobility shift assay (EMSA) was used to analyze the impact of the methylation of CpG sites on transcription factors. Human lens epithelial B-3 (HLE B-3) Cells were treated with demethylation agent zebularine in the concentrations of 0 (PBS as control), 10 

SUBMITTER: Liu X 

PROVIDER: S-EPMC4979130 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature