MiR-34a(-/-) mice are susceptible to diet-induced obesity.

ABSTRACT:

Objective

MicroRNA (miR)-34a regulates inflammatory pathways, and increased transcripts have been observed in serum and subcutaneous adipose of subjects who have obesity and type 2 diabetes. Therefore, the role of miR-34a in adipose tissue inflammation and lipid metabolism in murine diet-induced obesity was investigated.

Methods

Wild-type (WT) and miR-34a(-/-) mice were fed chow or high-fat diet (HFD) for 24 weeks. WT and miR-34a(-/-) bone marrow-derived macrophages were cultured in vitro with macrophage colony-stimulating factor (M-CSF). Brown and white preadipocytes were cultured from the stromal vascular fraction (SVF) of intrascapular brown and epididymal white adipose tissue (eWAT), with rosiglitazone.

Results

HFD-fed miR-34a(-/-) mice were significantly heavier

SUBMITTER: Lavery CA 

PROVIDER: S-EPMC4979678 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature