Protein Adsorption as a Key Mediator in the Nanotopographical Control of Cell Behavior.

ABSTRACT: Surface nanotopography is widely employed to control cell behavior and in particular controlled disorder has been shown to be important in cell differentiation/maturation. However, extracellular matrix proteins, such as fibronectin (FN), initially adsorbed on a biomaterial surface are known to mediate the interaction of synthetic materials with cells. In this work, we examine the effect of nanotopography on cell behavior through this adsorbed layer of adhesive proteins using a nanostructured polycarbonate surface comprising 150 nm-diameter pits originally defined using electron beam lithography. We address the effect of this nanopitted surface on FN adsorption and subsequently on cell morphology and behavior using C2C12 myoblasts. Wettability measurements and atomic force microscopy imagin

SUBMITTER: Ngandu Mpoyi E 

PROVIDER: S-EPMC4980054 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature