Production and characterization of a new antibacterial peptide obtained from Aeribacillus pallidus SAT4.

ABSTRACT: A novel thermophilic bacterial strain of the genus Aeribacillus was isolated from Thar Dessert Pakistan. This strain showed significant antibacterial activity against Micrococcus luteus, Staphylococcus aureus, and Pseudomonasaeruginosa. The strain coded as 'SAT4' resembled with Aeribacillus pallidus in the morphological, biochemical and molecular tests. The production of antibacterial metabolites by SAT4 was optimized. These active metabolites were precipitated by 50% ammonium sulphate and purified through sephadex G-75 gel permeation chromatography and reverse phase HPLC. The molecular weight of 37 kDa was examined by SDS-PAGE. The structural elucidation of the purified product was studied by FTIR, ¹H and ¹³C NMR. The X-ray d

SUBMITTER: Muhammad SA 

PROVIDER: S-EPMC4980744 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature