Metabolic activity in dormant conidia of Aspergillus niger and developmental changes during conidial outgrowth.

ABSTRACT: The early stages of development of Aspergillus niger conidia during outgrowth were explored by combining genome-wide gene expression analysis (RNAseq), proteomics, Warburg manometry and uptake studies. Resting conidia suspended in water were demonstrated for the first time to be metabolically active as low levels of oxygen uptake and the generation of carbon dioxide were detected, suggesting that low-level respiratory metabolism occurs in conidia for maintenance. Upon triggering of spore germination, generation of CO2 increased dramatically. For a short period, which coincided with mobilisation of the intracellular polyol, trehalose, there was no increase in uptake of O2 indicating that trehalose was metabolised by fermentation. Data from genome-wide mRNA profiling showed the presence of t

SUBMITTER: Novodvorska M 

PROVIDER: S-EPMC4981222 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature