Effects of Venous Superdrainage and Arterial Supercharging on Dorsal Perforator Flap in a Rat Model.

ABSTRACT:

Objective

To comparatively assess the effects of venous superdrainage and arterial supercharging on dorsal perforator flap survival.

Materials and methods

Sixty male Sprague-Dawley rats (450-550g) were randomly divided into three groups (n = 20), including control group (Control) and experimental groups A (venous superdrainage, Exp. A) and B (arterial supercharging, Exp. B). At postoperative day 7, survival areas of the flaps were evaluated and all animals underwent angiography. Laser Doppler was used to evaluate flap perfusion from 0h to 7days after surgery. Histology with hematoxylin and eosin staining was used to count microvessels. Tissue of "Choke vessels"was excised for quantification of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF)

SUBMITTER: Zheng J 

PROVIDER: S-EPMC4981354 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature