PCR detection of Burkholderia multivorans in water and soil samples.

ABSTRACT:

Background

Although semi-selective growth media have been developed for the isolation of Burkholderia cepacia complex bacteria from the environment, thus far Burkholderia multivorans has rarely been isolated from such samples. Because environmental B. multivorans isolates mainly originate from water samples, we hypothesized that water rather than soil is its most likely environmental niche. The aim of the present study was to assess the occurrence of B. multivorans in water samples from Flanders (Belgium) using a fast, culture-independent PCR assay.

Results

A nested PCR approach was used to achieve high sensitivity, and specificity was confirmed by sequencing the resulting amplicons. B. multivorans was detected in 11 % of the water samples (n = 112) and 92 % of the soil samp

SUBMITTER: Peeters C 

PROVIDER: S-EPMC4981952 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature