Insect transformation with piggyBac: getting the number of injections just right.

ABSTRACT: The insertion of exogenous genetic cargo into insects using transposable elements is a powerful research tool with potential applications in meeting food security and public health challenges facing humanity. piggyBac is the transposable element most commonly utilized for insect germline transformation. The described efficiency of this process is variable in the published literature, and a comprehensive review of transformation efficiency in insects is lacking. This study compared and contrasted all available published data with a comprehensive data set provided by a biotechnology group specializing in insect transformation. Based on analysis of these data, with particular focus on the more complete observational data from the biotechnology group, we designed a decision tool to aid researc

SUBMITTER: Gregory M 

PROVIDER: S-EPMC4982070 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature