Direct PCR of indigenous and invasive mosquito species: a time- and cost-effective technique of mosquito barcoding.

ABSTRACT: Millions of people die each year as a result of pathogens transmitted by mosquitoes. However, the morphological identification of mosquito species can be difficult even for experts. The identification of morphologically indistinguishable species, such as members of the Anopheles maculipennis complex (Diptera: Culicidae), and possible hybrids, such as Culex pipiens pipiens/Culex pipiens molestus (Diptera: Culicidae), presents a major problem. In addition, the detection and discrimination of newly introduced species can be challenging, particularly to researchers without previous experience. Because of their medical importance, the clear identification of all relevant mosquito species is essential. Using the direct polymerase chain reaction (PCR) method described here, DNA amplification with

SUBMITTER: Werblow A 

PROVIDER: S-EPMC4982094 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature