Comparison of pre-processing methods for multiplex bead-based immunoassays.

ABSTRACT:

Background

High throughput protein expression studies can be performed using bead-based protein immunoassays, such as the Luminex® xMAP® technology. Technical variability is inherent to these experiments and may lead to systematic bias and reduced power. To reduce technical variability, data pre-processing is performed. However, no recommendations exist for the pre-processing of Luminex® xMAP® data.

Results

We compared 37 different data pre-processing combinations of transformation and normalization methods in 42 samples on 384 analytes obtained from a multiplex immunoassay based on the Luminex® xMAP® technology. We evaluated the performance of each pre-processing approach with 6 different performance criteria. Three performance criteria were plots. All plots were evaluated

SUBMITTER: Rausch TK 

PROVIDER: S-EPMC4982217 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature