The influence of trematode parasite burden on gene expression in a mammalian host.

ABSTRACT:

Background

Parasites can profoundly impact their hosts and are responsible for a plethora of debilitating diseases. To identify global changes in host gene expression related to parasite infection, we sequenced, assembled, and annotated the liver transcriptomes of Balb/cj mice infected with the trematode parasite Schistosoma mansoni and compared the results to uninfected mice. We used two different methodologies (i.e. de novo and reference guided) to evaluate the influence of parasite sequences on host transcriptome assembly.

Results

Our results demonstrate that the choice of assembly methodology significantly impacted the proportion of parasitic reads detected from the host library, yet the presence of non-target (xenobiotic) sequences did not create significant structural

SUBMITTER: Wijayawardena BK 

PROVIDER: S-EPMC4982272 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature