Glial restricted precursors maintain their permissive properties after long-term expansion but not following exposure to pro-inflammatory factors.

ABSTRACT: Glial restricted precursors (GRP) are a promising cellular source for transplantation therapy of spinal cord injury (SCI), capable of creating a permissive environment for axonal growth and regeneration. However, there are several issues regarding the nature of their permissive properties that remain unexplored. For example, cellular transplantation strategies for spinal cord repair require the preparation of a large number of cells, but it is unknown whether the permissive properties of GRP are maintained following the process of in vitro expansion. We used rat GRP isolated from the embryonic day 13.5 spinal cord to compare the properties of early (10-20 days) and late (120-140 days) passage GRP. We found that late passage GRP showed comparable effects on neurite outgrowth of adult rat DR

SUBMITTER: Hayakawa K 

PROVIDER: S-EPMC4982471 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature