Comparative transcriptome analysis by RNAseq of necrotic enteritis Clostridium perfringens during in vivo colonization and in vitro conditions.

ABSTRACT:

Background

Necrotic enteritis (NE) caused by netB-positive type A Clostridium perfringens is an important bacterial disease of poultry. Through its complex regulatory system, C. perfringens orchestrates the expression of a collection of toxins and extracellular enzymes that are crucial for the development of the disease; environmental conditions play an important role in their regulation. In this study, and for the first time, global transcriptomic analysis was performed on ligated intestinal loops in chickens colonized with a netB-positive C. perfringens strain, as well as the same strain propagated in vitro under various nutritional and environmental conditions.

Results

Analysis of the respective pathogen transcriptomes revealed up to 673 genes that were significantly expr

SUBMITTER: Parreira VR 

PROVIDER: S-EPMC4983038 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature