A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations.

ABSTRACT:

Background

Acetic acid, released during hydrolysis of lignocellulosic feedstocks for second generation bioethanol production, inhibits yeast growth and alcoholic fermentation. Yeast biomass generated in a propagation step that precedes ethanol production should therefore express a high and constitutive level of acetic acid tolerance before introduction into lignocellulosic hydrolysates. However, earlier laboratory evolution strategies for increasing acetic acid tolerance of Saccharomyces cerevisiae, based on prolonged cultivation in the presence of acetic acid, selected for inducible rather than constitutive tolerance to this inhibitor.

Results

Preadaptation in the presence of acetic acid was shown to strongly increase the fraction of yeast cells that could initiate growth i

SUBMITTER: Gonzalez-Ramos D 

PROVIDER: S-EPMC4983051 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature