Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency.

ABSTRACT: Embryonic stem cells (ESCs) are cell lines derived from the mammalian pre-implantation embryo. Here we assess the impact of derivation and culture conditions on both functional potency and ESC transcriptional identity. Individual ESCs cultured in either two small-molecule inhibitors (2i) or with knockout serum replacement (KOSR), but not serum, can generate high-level chimeras regardless of how these cells were derived. ESCs cultured in these conditions showed a transcriptional correlation with early pre-implantation embryos (E1.5-E3.5) and contributed to development from the 2-cell stage. Conversely, the transcriptome of serum-cultured ESCs correlated with later stages of development (E4.5), at which point embryonic cells are more restricted in their developmental potential. Thus, ESC cul

SUBMITTER: Martin Gonzalez J 

PROVIDER: S-EPMC4983099 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature