Evaluation of coverage, retention patterns, and selectivity of seven liquid chromatographic methods for metabolomics.

ABSTRACT: Liquid chromatography-mass spectrometry-based metabolomics studies require highly selective and efficient chromatographic techniques. Typically employed reversed-phase (RP) methods fail to target polar metabolites, but the introduction of hydrophilic interaction liquid chromatography (HILIC) is slow due to perceived issues of reproducibility and ruggedness and a limited understanding of the complex retention mechanisms. In this study, we present a comparison of the chromatographic performance of a traditional RP-C18 column with zwitterionic, amide-, alkyl diol-, and aminoalkyl-based HILIC and mixed-mode columns. Our metabolite library represents one of the largest analyte sets available and consists of 764 authentic metabolite standards, including amino acids, nucleotides, sugars, and othe

SUBMITTER: Wernisch S 

PROVIDER: S-EPMC4983217 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature