Bioprocess monitoring: minimizing sample matrix effects for total protein quantification with bicinchoninic acid assay.

ABSTRACT: Determining total protein content is a routine operation in many laboratories. Despite substantial work on assay optimization interferences, the widely used bicinchoninic acid (BCA) assay remains widely recognized for its robustness. Especially in the field of bioprocess engineering the inaccuracy caused by interfering substances remains hardly predictable and not well understood. Since the introduction of the assay, sample pre-treatment by trichloroacetic acid (TCA) precipitation has been indicated as necessary and sufficient to minimize interferences. However, the sample matrix in cultivation media is not only highly complex but also dynamically changing over process time in terms of qualitative and quantitative composition. A significant misestimation of the total protein concentration

SUBMITTER: Reichelt WN 

PROVIDER: S-EPMC4983285 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature