A proteomic evaluation of urinary changes associated with cardiopulmonary bypass.

ABSTRACT:

Background

The urinary proteome of patients undergoing cardiopulmonary bypass (CPB) may provide important insights into systemic and renal changes associated with the procedure. Such information may ultimately provide a basis to differentiate changes or properties associated with the development of acute kidney injury. While mass spectrometry (MS) analysis offers the potential for in-depth compositional analysis it is often limited in coverage and relative quantitation capacity. The aim of this study was to develop a process flow for the preparation and comparison of the intraoperative urinary proteome.

Methods

Urines were collected from patients at the start of CPB and 1-h into CPB. Pooled samples (n = 5) from each time point were processed using a modified Filter Assisted

SUBMITTER: Dwivedi RC 

PROVIDER: S-EPMC4983784 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature