Disparate response of articular- and auricular-derived chondrocytes to oxygen tension.

ABSTRACT:

Purpose/aim

To determine the effect of reduced (5%) oxygen tension on chondrogenesis of auricular-derived chondrocytes. Currently, many cell and tissue culture experiments are performed at 20% oxygen with 5% carbon dioxide. Few cells in the body are subjected to this supra-physiological oxygen tension. Chondrocytes and their mesenchymal progenitors are widely reported to have greater chondrogenic expression when cultured at low, more physiological, oxygen tension (1-7%). Although generally accepted, there is still some controversy, and different culture methods, species, and outcome metrics cloud the field. These results are, however, articular chondrocyte biased and have not been reported for auricular-derived chondrocytes.

Materials and methods

Auricular and articular chon

SUBMITTER: Kean TJ 

PROVIDER: S-EPMC4984267 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature