Environmental Sequencing Provides Reasonable Estimates of the Relative Abundance of Specific Picoeukaryotes.

ABSTRACT:

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High-throughput sequencing (HTS) is revolutionizing environmental surveys of microbial diversity in the three domains of life by providing detailed information on which taxa are present in microbial assemblages. However, it is still unclear how the relative abundance of specific taxa gathered by HTS correlates with cell abundances. Here, we quantified the relative cell abundance of 6 picoeukaryotic taxa in 13 planktonic samples from 6 European coastal sites using epifluorescence microscopy on tyramide signal amplification-fluorescence in situ hybridization preparations. These relative abundance values were then compared with HTS data obtained in three separate molecular surveys: 454 sequencing of the V4 region of the 18S ribosomal DNA (rDNA) using DNA and RNA extracts (D

SUBMITTER: Giner CR 

PROVIDER: S-EPMC4984273 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature