Pichia pastoris Exhibits High Viability and a Low Maintenance Energy Requirement at Near-Zero Specific Growth Rates.

ABSTRACT:

Unlabelled

The yeast Pichia pastoris is a widely used host for recombinant protein production. Understanding its physiology at extremely low growth rates is a first step in the direction of decoupling product formation from cellular growth and therefore of biotechnological relevance. Retentostat cultivation is an excellent tool for studying microbes at extremely low specific growth rates but has so far not been implemented for P. pastoris Retentostat feeding regimes were based on the maintenance energy requirement (mS) and maximum biomass yield on glucose (YX /S (max)) estimated from steady-state glucose-limited chemostat cultures. Aerobic retentostat cultivation enabled reproducible, smooth transitions from a specific growth rate (μ) of 0.025 h(-1) to near-zero specific growth rat

SUBMITTER: Rebnegger C 

PROVIDER: S-EPMC4984280 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature