Association of CpG island methylator phenotype and EREG/AREG methylation and expression in colorectal cancer.

ABSTRACT:

Background

High EREG and AREG expression, and left-sided primary tumours are associated with superior efficacy of anti-epidermal growth factor receptor (EGFR) therapy in metastatic colorectal cancer (CRC), but a unifying explanation of these findings is lacking.

Methods

RNA-seq, gene expression arrays, and DNA methylation profiling were completed on 179 CRC tumours. Results were validated using independent The Cancer Genome Atlas data sets. An independent cohort of 198 KRAS wild-type metastatic CRC tumours was tested for CpG island methylator phenotype (CIMP) status, and progression-free survival (PFS) with the first anti-EGFR regimen was retrospectively determined.

Results

EREG and AREG expression was highly inversely correlated with methylation and was inversely ass

SUBMITTER: Lee MS 

PROVIDER: S-EPMC4984478 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature