COPD Exacerbation Biomarkers Validated Using Multiple Reaction Monitoring Mass Spectrometry.

ABSTRACT:

Background

Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) result in considerable morbidity and mortality. However, there are no objective biomarkers to diagnose AECOPD.

Methods

We used multiple reaction monitoring mass spectrometry to quantify 129 distinct proteins in plasma samples from patients with COPD. This analytical approach was first performed in a biomarker cohort of patients hospitalized with AECOPD (Cohort A, n = 72). Proteins differentially expressed between AECOPD and convalescent states were chosen using a false discovery rate <0.01 and fold change >1.2. Protein selection and classifier building were performed using an elastic net logistic regression model. The performance of the biomarker panel was then tested in two independent AECOPD c

SUBMITTER: Leung JM 

PROVIDER: S-EPMC4985129 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature