Constitutive aneuploidy and genomic instability in the single-celled eukaryote Giardia intestinalis.

ABSTRACT: Giardia intestinalis is an important single-celled human pathogen. Interestingly, this organism has two equal-sized transcriptionally active nuclei, each considered diploid. By evaluating condensed chromosome numbers and visualizing homologous chromosomes by fluorescent in situ hybridization, we determined that the Giardia cells are constitutively aneuploid. We observed karyotype inter-and intra-population heterogeneity in eight cell lines from two clinical isolates, suggesting constant karyotype evolution during in vitro cultivation. High levels of chromosomal instability and frequent mitotic missegregations observed in four cell lines correlated with a proliferative disadvantage and growth retardation. Other cell lines, although derived from the same clinical isolate, revealed a stable y

SUBMITTER: Tumova P 

PROVIDER: S-EPMC4985590 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature