Expanding the molecular toolkit for the homoacetogen Clostridium ljungdahlii.

ABSTRACT: Increasing interest in homoacetogenic bacteria for the production of biochemicals and biofuels requisites the development of new genetic tools for these atypical production organisms. An attractive host for the conversion of synthesis gas or electricity into multi-carbon compounds is Clostridium ljungdahlii. So far only limited achievements in modifying this organism towards the production of industrially relevant compounds have been made. Therefore, there is still a strong need for developing new and optimizing existing genetic tools to efficiently access its metabolism. Here, we report on the development of a stable and reproducible transformation protocol that is applicable to C. ljungdahlii and several other clostridial species. Further, we demonstrate the functionality of a temperatur

SUBMITTER: Molitor B 

PROVIDER: S-EPMC4985741 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature