Pasteurella multocida Bacteremia With Associated Knee Arthroplasty Infection in an 80-Year-Old Caucasian Man.

ABSTRACT:

Objectives

To identify the gram-negative rods grown from blood cultures and a right-knee fluid aspirate from an 80-year-old caucasian man who had undergone a total right knee arthroplastic procedure 6 years ago, and to assess the genetic similarity between the 2 isolates.

Methods

We used 3 different approaches: biochemical testing, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and 16S ribosomal RNA (rRNA) gene sequencing.

Results

The 3 methods identified the gram-negative rods as Pasteurella multocida; 16S rRNA gene sequencing further identified the organisms as P. multocida subsp. septica.

Conclusion

A concordant identification of P. multocida was observed using biochemical testing, mass spectrometry, and 16S rRNA ge

SUBMITTER: Arbefeville S 

PROVIDER: S-EPMC4985777 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature