A strategy to identify housekeeping genes suitable for analysis in breast cancer diseases.

ABSTRACT:

Background

The selection of suitable internal control genes is crucial for proper interpretation of real-time PCR data. Here we outline a strategy to identify housekeeping genes that could serve as suitable internal control for comparative analyses of gene expression data in breast cancer cell lines and tissues obtained by high throughput sequencing and quantitative real-time PCR (qRT-PCR).

Methods

The strategy proposed includes the large-scale screening of potential candidate reference genes from RNA-seq data as well as their validation by qRT-PCR, and careful examination of reference data from the International Cancer Genome Consortium, The Cancer Genome Atlas and Gene Expression Omnibus repositories.

Results

The identified set of reference genes, also called novel

SUBMITTER: Tilli TM 

PROVIDER: S-EPMC4986254 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature