Development and Validation of a GC-FID Method for Diagnosis of Methylmalonic Acidemia.

ABSTRACT:

Background

Urinary organic acids are water-soluble intermediates and end products of the metabolism of amino acids, carbohydrates, lipids, and a number of other metabolic processes. In the hereditary diseases known as organic acidurias, an enzyme or co-factor defect in a metabolic pathway leads to the accumulation and increased excretion of one or more of these acidic metabolites. Gas chromatography is the most commonly-used technology to separate and identify these metabolites. In this report the analytical conditions for the determination of methylmalonic acid using a gas chromatography/flame ionization detector (GC-FID) are studied with the aim to establish a method to analyze organic acids in human urine.

Methods

Studies included the GC-FID method development, the condit

SUBMITTER: Keyfi F 

PROVIDER: S-EPMC4986268 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature