Multistage Mass Spectrometry of Phospholipids using Collision-Induced Dissociation (CID) and Metastable Atom-Activated Dissociation (MAD).

ABSTRACT: We herein demonstrate an approach to gas phase ion manipulation that provides MS³-level CID spectra of phospholipid radical cations that are almost independent of the original charging adduct ions. In the MS² He-MAD spectra of the protonated, sodiated and potassiated adducts of POPC, the different adducts induce different primary fragmentation pathways and provide significantly different spectra, as is commonly observed by other activation methods. In separate experiments, the even-electron adduct ions ([M+H]⁺, [M+Na]⁺, [M+K]⁺) of 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) were first converted to radical cations [POPC]^+• by using helium metastable atom-activated dissociation (He-MAD) to eject the charging add

SUBMITTER: Li P 

PROVIDER: S-EPMC4987003 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature