Autophagy mediates epithelial cytoprotection in eosinophilic oesophagitis.

ABSTRACT:

Objective

The influence of eosinophilic oesophagitis (EoE)-associated inflammation upon oesophageal epithelial biology remains poorly understood. We investigated the functional role of autophagy in oesophageal epithelial cells (keratinocytes) exposed to the inflammatory EoE milieu.

Design

Functional consequences of genetic or pharmacological autophagy inhibition were assessed in endoscopic oesophageal biopsies, human oesophageal keratinocytes, single cell-derived ex vivo murine oesophageal organoids as well as a murine model recapitulating EoE-like inflammation and basal cell hyperplasia. Gene expression, morphological and functional characterisation of autophagy and oxidative stress were performed by transmission electron microscopy, immunostaining, immunoblotting, live cel

SUBMITTER: Whelan KA 

PROVIDER: S-EPMC4987278 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature