Systematic evaluation of sericin protein as a substitute for fetal bovine serum in cell culture.

ABSTRACT: Fetal bovine serum (FBS) shows obvious deficiencies in cell culture, such as low batch to batch consistency, adventitious biological contaminant risk, and high cost, which severely limit the development of the cell culture industry. Sericin protein derived from the silkworm cocoon has become increasingly popular due to its diverse and beneficial cell culture characteristics. However, systematic evaluation of sericin as a substitute for FBS in cell culture medium remains limited. In this study, we conducted cellular morphological, physiological, and transcriptomic evaluation on three widely used mammalian cells. Compared with cells cultured in the control, those cultured in sericin-substitute medium showed similar cellular morphology, similar or higher cellular overall survival, lower popul

SUBMITTER: Liu L 

PROVIDER: S-EPMC4987615 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature