Ontology highlight
ABSTRACT:
SUBMITTER: Nishitani C
PROVIDER: S-EPMC4987624 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Scientific reports 20160817
Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system. Four guide RNAs (gRNAs) were designed and stably transformed with Cas9 separately i ...[more]